Fig. 1: Mul-A suppresses the diffrentiation of BMMs in vitro. | Cell Death Discovery

Fig. 1: Mul-A suppresses the diffrentiation of BMMs in vitro.

From: Restrained Mitf-associated autophagy by Mulberroside A ameliorates osteoclastogenesis and counteracts OVX-Induced osteoporosis in mice

Fig. 1

A The chemical structure of Mul-A. B, C The activity of BMMs was assessed at different concentrations of Mul-A. D BMMs were cultured with 0, 20, 40, and 80 μM Mul-A for 6 days under the conditions of 30 ng/mL M-CSF and 50 ng/mL RANKL. Double staining with Trap and phalloidin was performed (n = 5). E–G Quantitative analysis was conducted to evaluate the number of Trap-positive multinuclear cells and the length of F-actin per well (n = 5). H BMMs were treated with 80 μM Mul-A in the early (1-3 D), middle (3-5 D), and late (5-7 D) stages of osteoclast differentiation. Double staining with Trap and phalloidin was performed (n = 5). I–K Quantitative analysis was performed to measure the number of Trap-positive multinuclear cells and the length of F-actin per well (n = 5). Scale bar = 200 μm. The control group was treated with an equivalent amount of DMSO. Data were presented as the median and interquartile range (IQR). *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page