Fig. 2: Mul-A attenuates the bone resorption capacity and specific gene expression of osteoclasts. | Cell Death Discovery

Fig. 2: Mul-A attenuates the bone resorption capacity and specific gene expression of osteoclasts.

From: Restrained Mitf-associated autophagy by Mulberroside A ameliorates osteoclastogenesis and counteracts OVX-Induced osteoporosis in mice

Fig. 2

A BMMs were cultured with 30 ng/mL M-CSF and 50 ng/mL RANKL for 6 days. Following the maturation of osteoclasts, cells were subsequently treated with various concentrations of Mul-A (0, 20, 40, 80 μM) for an additional 4-5 days. The bovine bone slices were collected for scanning electron microscopy analysis. B Quantitative analysis of bone resorption capacity per cell (n = 5). C–H BMMs were treated with 50 ng/mL RANKL and 30 ng/mL M-CSF in the presence or absence of 80 μM Mul-A for 0, 3, 5, and 7 days. The mRNA levels of osteoclast-related genes were quantitatively analyzed using quantitative real-time PCR (n = 5). I BMMs were treated with 50 ng/mL RANKL and 30 ng/mL M-CSF in the presence or absence of 80 μM Mul-A for 0, 3, 5, and 7 days. The control group was treated with the equivalent amount of DMSO. The protein levels of Nfatc1, Ctsk, and C-Fos were examined by western blotting. J–L Quantitative evaluation of the relative grayscale intensity of protein bands (n = 3). Scale bar = 50 μm. The control group was added with an equivalent DMSO. Data were presented as the median and interquartile range (IQR). *P < 0.05, **P < 0.01, ***P < 0.001.

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