Fig. 5: Effects of Trx-1 on expression of PINK1, Parkin and cathepsin D in the SNpc in MPTP-treated mice.
From: Thioredoxin-1 decreases alpha-synuclein induced by MPTP through promoting autophagy-lysosome pathway
The expression of PINK1 and Parkin in the SNpc were detected by Western blot analysis after administration with MPTP. The MPTP-increased PINK1 was reduced in Trx-1 overexpressing transgenic (Tg) mice A and was further increased in Trx-1 knockdown in the SNpc in mice B. The MPTP-increased Parkin was reduced in Trx-1 overexpressing transgenic (Tg) mice C and was further increased in Trx-1 knockdown in the SNpc in mice D. The expression of cathepsin D in the SNpc in mice treated with MPTP was detected by Western blot analysis. The MPTP-decreased cathepsin D was restored in Trx-1 overexpressing transgenic (Tg) mice E and was further decreased in Trx-1 knockdown in the SNpc in mice F. Each bar represents the mean ± SEM (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, statistically significant.
