Fig. 3: PARVB expression was upregulated, while the ILK-AKT related pathways were downregulated in the inner ears of Fscn2−/− mice at 8 weeks of age. | Cell Death Discovery

Fig. 3: PARVB expression was upregulated, while the ILK-AKT related pathways were downregulated in the inner ears of Fscn2−/− mice at 8 weeks of age.

From: Inhibition of the ILK-AKT pathway by upregulation of PARVB contributes to the cochlear cell death in Fascin2 gene knockout mice

Fig. 3

A The expression of PARVB in the spiral ganglion neurons (SGNs) in the cochleae of Fscn2+/+ and Fscn2−/− mice was localized by immunofluorescence histochemical analysis. Scale bar, 20 μm. B The expression of p-AKT was detected by immunohistochemical staining in the cochleae of Fscn2+/+ and Fscn2−/−mice. The images are representative of three experiments. Scale bar, 50 μm. C The levels of PARVB, ILK, p-ILK, AKT, p-AKT, cleaved-Caspase9 and Bcl-2 were detected by Western blot in the inner ears of Fscn2+/+ and Fscn2−/− mice at 8 weeks of age. D–I The six panels on the right side show the results of densitometric analysis of the bands on Western blots. Data are represented as means ± SEM. *P < 0.05; **P < 0.01.

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