Fig. 2: Garcinol treatment restores H2O2-induced abnormal cell fate of BMSCs. | Cell Death Discovery

Fig. 2: Garcinol treatment restores H2O2-induced abnormal cell fate of BMSCs.

From: Garcinol prevents oxidative stress-induced bone loss and dysfunction of BMSCs through NRF2-antioxidant signaling

Fig. 2

A The osteoblastic commitment of H2O2-treated BMSCs after Garcinol administration was determined by ALP staining. Scale bar=100 μm. B The mineralized depositions of BMSCs was measured by ARS staining. Scale bar=100 μm. C Measurements of mRNA levels of osteoblastic marker genes in BMSCs. D The adipogeneic differentiation of H2O2-treated BMSCs after Garcinol supplementation was quantified by ORO staining. Scale bar=50 μm. E The mRNA levels of key adipocyte-related genes were evaluated by qRT-PCR analysis. N = 4. The comparisons among groups were analyzed by ANOVA analysis. **P < 0.01, ***P < 0.001,  and ****P < 0.0001 vs. the CTL group. #P < 0.05, ##P < 0.01 ###P < 0.001 and ####P < 0.0001 vs. the H2O2 group. H2O2 hydrogen peroxide, ALP alkaline phosphatase, ARS alizarin red S, ORO oil red O, BMP4 bone morphogenetic protein 4, Runx2 runtrelated transcription factor 2, PPARγ peroxisome proliferator-activated receptor γ, Fabp4 fatty-acid-binding protein 4, C/EBPα CCAAT enhancer-binding protein α.

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