Fig. 6: Garcinol protects damaged BMSCs and bone loss from oxidative stress by activating NRF2-antioxidant signaling. | Cell Death Discovery

Fig. 6: Garcinol protects damaged BMSCs and bone loss from oxidative stress by activating NRF2-antioxidant signaling.

From: Garcinol prevents oxidative stress-induced bone loss and dysfunction of BMSCs through NRF2-antioxidant signaling

Fig. 6

A The cell viability of BMSCs from CTL group, H2O2 group, H2O2+Garcinol group, H2O2+Garcinol+siRNA-NC, H2O2+Garcinol+siRNA-NRF2 group, was confirmed by CCK-8. B Trypan blue exclusion assay was utilized to quantify the cell death of BMSCs from CTL group, H2O2 group, H2O2+Garcinol group, H2O2+Garcinol+siRNA-NC, H2O2+Garcinol+siRNA-NRF2 group. C The quantification of TUNEL staining. D ARS staining and quantitative analysis. Scale bar=100 μm. E ALP staining and quantity of mineralization. Scale bar=100 μm. F ORO staining and quantitative analysis of adipocytes. Scale bar=50 μm. N = 4, The comparisons among groups were analyzed by ANOVA analysis. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. H2O2 hydrogen peroxide, NRF2 nuclear factor erythroid 2-related factor 2, ARS alizarin red S, ALP alkaline phosphatase.

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