Fig. 5: The absence of AIM2 results in increased activation of JNK and ERK pathways. | Cell Death Discovery

Fig. 5: The absence of AIM2 results in increased activation of JNK and ERK pathways.

From: AIM2 regulates autophagy to mitigate oxidative stress in aged mice with acute liver injury

Fig. 5

A, B WT and AIM2−/− mice aged 30–32 weeks were administered either PBS or APAP for 1, 2, 3 h, and C, D for 24 h. E, F WT and AIM2−/− mice aged 6–8 weeks were supplemented with PBS or APAP for 24 h. Protein expression was analyzed using western blotting, and phosphorylated proteins were normalized to total proteins. A representative Western blot from three independent experiments is shown. Immunoblot analysis of PCNA in liver homogenates was conducted (G), and quantification of Evans blue dye extravasation was performed at 24 h after APAP injection (H). The number of mice in each group ranged from 9 to 10, and the statistical significance levels are indicated as follows: *P < 0.05, **P < 0.01, and ***P < 0.001.

Back to article page