Fig. 6: AIM2 deficiency promotes ERK signaling activation in macrophages and inhibits autophagy pathway activation in hepatocytes.
From: AIM2 regulates autophagy to mitigate oxidative stress in aged mice with acute liver injury
A Western blot analysis of cleaved caspase-3 and 8 in primary hepatocytes from WT and AIM2−/− mice aged 30 weeks stimulated with APAP (10 mM). B Primary hepatocytes from 30-week-old mice were treated with 10 mM APAP for 6 and 24 h, and culture supernatants were collected for AST and ALT measurement. C, D Bone marrow-derived macrophages (BMDMs) from 30-week-old mice were treated with HMGB1 (200 ng/mL), and cell proteins were collected for Western blotting. E–H TAMH cells were transfected with poly(dA:dT) or Flag-AIM2 for 24 h and then stimulated with 10 mM APAP. Cell lysates were obtained for Western blot analysis. Data represent the mean ± SEM of three independent experiments. **P < 0.01; ***P < 0.001; ****P < 0.0001.
