Fig. 1: In vitro and In vivo mouse postovulatory oocyte death kinetics. | Cell Death Discovery

Fig. 1: In vitro and In vivo mouse postovulatory oocyte death kinetics.

From: Diminished NAD+ levels and activation of retrotransposons promote postovulatory aged oocyte (POAO) death

Fig. 1

a, e Schematic representation of postovulatory oocyte ageing. b POYO and POAO stained with trypan blue and FDA. c Quantification of trypan blue positive (TB +ve) and blebbed oocytes from in vitro maturation (d) POAO deathkinetics. f In vivo POYO and POAO stained with trypan blue and FDA. g Quantification of trypan blue +ve and blebbed oocytes from in vivo maturation. h Quantification of % live and dead oocytes from in vivo maturation. More than 500 matured oocytes used to quantify the in vitro death. 150 to 200 oocytes used to quantify the in vivo death. Error bars show mean ± s.e.m **P ≤ 0.008, unpaired t-tests. Scale bars = 100 μm.

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