Fig. 7: Evolutionary conservation of sodium pyruvate-dependent delayed POAO death.

a POYO and POAO nuclei were immunostained for ORF1 (green), γH2AX (red) and DNA (magenta). b Quantification of ORF1 and γH2AX colocalized nuclei in respective ages. c Schematic representation of goat oocyte IVM followed by POAO with or without AZT. d POAO with or without AZT stained for FDA (green). e Quantification of % live POAO with or without AZT at 72 h. f Schematic representation of goat oocyte IVM followed by POAO with or without sodium pyruvate. g POAO with or without sodium pyruvate stained for FDA (green). h Quantification of % live POAO with or without sodium pyruvate at 72 h. i Schematic representation of goat oocyte IVM followed by POAO with or without sodium pyruvate and IVF, embryo maturation. j Bright field images of goat embryo (k) Quantification of embryo development of goat POAO with or without sodium pyruvate. 20 to 25 oocytes were analyzed for localizations and colocalizations. Error bars show mean ± s.e.m ***P ≤ 0.0001, **P ≤ 0.004, *P ≤ 0.03, unpaired t-tests. Scale bars = 100 μm.