Fig. 3: SULF1 contributed to CAFs-induced migration and invasion of GC cells.

A The top 10 enriched signaling pathways of SULF1 using GSEA analysis. B. Western blotting to detected the expression of EMT markers in HGC27 and AGS cells from indicated groups. Immunofluorescence stained E-cadherin and vimentin to detected the EMT process in HGC27 (C) and AGS (D) cells from indicated groups. Representative images of wound-healing (E) and transwell (F) assays in HGC27 cells from indicated groups. Quantitative analysis results of wound-healing (G) and transwell (H) assays in HGC27 cells from indicated groups. Quantitative analysis results of wound-healing (I) and transwell (J) assays in AGS cells from indicated groups. Representative images of wound-healing (K) and transwell (L) assays in AGS cells from indicated groups.