Fig. 1: Results of mouse ACF CRISPR knockout library screening conducted to identify which genes can affect migration in PDAC.
From: Roflumilast inhibits tumor growth and migration in STK11/LKB1 deficient pancreatic cancer
a Overview of the CRISPR/Cas9 screening procedure is provided in the Methods section. b Plot showing the enrichment of the top candidate genes in the accelerated migration group (blue dots) identified by PinAPL.py - compared to other genes in the library (gray dots) after selection. c The number of genes with zero, one, two, three, or four significantly enriched sgRNAs (p < 0.05 for three separate screening) targeting that gene in the accelerated migration group. Genes are categorized by the number of enriched sgRNAs, as indicated in the colored bubbles adjacent to each bar. d STK11 have multiple enriched sgRNAs in the accelerated migration cells (Control Group: library cells before screening; Treatment Group: library cells after screening). e Basal expression of STK11 in 10 different PDAC cell lines was detected by western blot. GAPDH was used as a loading control.
