Fig. 5: The RNA sequencing and analysis of the GO pathway revealed that the significant gene expression change was induced by loss of STK11 in the TB 32047 and PANC-1 samples.

a, b GO functional classification of the DEGs in TB 32047 and PANC-1 cells (STK11^KO vs. Control). The distributions are summarized in three main categories: BP, MF, and CC. The x axis indicates the number of genes in each category, and the y axis indicates different GO terms. c, d Volcano plot representation of the RNA-seq results showing the number of genes with significantly altered expression after STK11 knockout in TB 32047 and PANC-1 cells. In the volcano graph, non-significant genes are shown in gray, significant genes (|log₂FC|>1) are highlighted in green, p < 0.05 genes are highlighted in blue, and genes with significant p-values and log₂FC values are highlighted in red. a–d were plotted by https://www.bioinformatics.com.cn (last accessed on 31 Oct 202), an online platform for data analysis and visualization. e Venn diagram showing the overlap between sets of differentially expressed transcripts (|log₂FC|>1, p < 0.05) in TB 32047 and PANC-1 samples. f Western blot analysis of Sox17, IL33, PDE4D, and PDE10A in STK11^KO TB 32047 and PANC-1 samples. GAPDH was used as the loading control. The observation of multiple bands in this analysis is due to the existence of various isoforms for the enzymes PDE4B, PDE4D, and PDE10A.