Fig. 4: Loss of USP7 inhibited OS cell proliferation and migration.
From: mTORC1 accelerates osteosarcoma progression via m6A-dependent stabilization of USP7 mRNA
The relative cell proliferation in shCtrl, shUSP7#1 or shUSP7#2 transfected MG63 (A) and U2OS (B) cells for 0-96 h. C, D The migration capacity and migrated cell number in shCtrl, shUSP7#1 or shUSP7#2 transfected MG63 for 24 h, scale bar: 50 µm. E, F The migration capacity and migrated cell number in shCtrl, shUSP7#1 or shUSP7#2 transfected U2OS for 24 h, scale bar: 50 µm. G, H TUNEL staining and TUNEL positive cells in shCtrl or shUSP7#2 transfected U2OS for 24 h, scale bar: 50 µm. I The Cyp19a1 and Cyp11a1 protein expression levels in shCtrl or shUSP7#2 transfected U2OS cells for 48 h. J The E2 content in shCtrl or shUSP7#2 transfected U2OS cells for 48 h. K, L The formation and quantification of ASC speck in U2OS cells transfected with shCtrl or shUSP7#2, scale bar: 50 µm. Arrows imply ASC speck. *P < 0.05, **P < 0.01, ***P < 0.001 vs control group.
