Fig. 4: Synergistic action of hypoxanthine and HPRT1 in promoting ESCC development. | Cell Death Discovery

Fig. 4: Synergistic action of hypoxanthine and HPRT1 in promoting ESCC development.

From: Purine salvage–associated metabolites as biomarkers for early diagnosis of esophageal squamous cell carcinoma: a diagnostic model–based study

Fig. 4

A Hypoxanthine promoted the proliferation of KYSE-30 and KYSE-450 cells. Two-way ANOVA test, **p < 0.01, ***p < 0.001. B Hypoxanthine stimulated the colony formation of KYSE-30 and KYSE-450 cells. C, D Establishment of HPRT1 stably silenced and overexpressed cell lines verified at the mRNA and protein expression level in ESCC cells (KYSE-30 and KYSE-450).Two-tailed unpaired t test, **p < 0.01, ***p < 0.001. E HPRT1 overexpression potentiated the proliferation-promoting effect of hypoxanthine in KYSE-30 and KYSE-450 cells. Two-way ANOVA test, **p < 0.01, ***p < 0.001. F HPRT1 overexpression increased the clonogenicity effects of hypoxanthine in KYSE-30 and KYSE-450 cells. G HPRT1 silencing with shRNAs diminished the pro-proliferative effects of hypoxanthine in ESCC cells. Two-way ANOVA test, **p < 0.05, **p < 0.01, ***p < 0.001. H Hypoxanthine concentration in cells with HPRT1 overexpression or silencing. Two-tailed unpaired t test, ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001. I Effect of hypoxanthine on the expression of the HPRT1 gene in KYSE-30 and KYSE-450 cells. Two-tailed unpaired t test, ns not significan. ESCC esophageal squamous cell carcinoma, shRNA short hairpin RNA.

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