Fig. 5: HPRT1 promoted the malignant proliferation of ESCC cells in vitro and in vivo. | Cell Death Discovery

Fig. 5: HPRT1 promoted the malignant proliferation of ESCC cells in vitro and in vivo.

From: Purine salvage–associated metabolites as biomarkers for early diagnosis of esophageal squamous cell carcinoma: a diagnostic model–based study

Fig. 5

A HPRT1 silencing with shRNAs inhibited the proliferation of KYSE-30 and KYSE-450 cells. Two-way ANOVA test, **p < 0.01, ***p < 0.001. B Silencing of HPRT1 with shRNAs inhibited the clonogenicity of KYSE-30 and KYSE-450 cells. Two-tailed unpaired t test, *p < 0.05, **p < 0.01. C, D In vivo growth of ESCC xenografts was inhibited by HPRT1 silenced sh3 (KYSE-30) and sh5 (KYSE-450) vs corresponding shNC. E Venn diagram showing HPRT1 silencing affects gene expression in KYSE-30 and KYSE-450 cells. F KEGG enrichment pathways of the DEGs in both HPRT1-silenced KYSE-30 and KYSE-450 cells. G, H HPRT1 suppressed the phosphorylation of the YAP protein in KYSE-30 and KYSE-450 cells. Two-tailed unpaired t test, ns, not significant, *p < 0.05, **p < 0.01. ESCC esophageal squamous cell carcinoma, shRNA short hairpin RNA, DEGs differentially expressed genes, KEGG Kyoto Encyclopedia of Genes and Genomes.

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