Fig. 2: The 25-HC regulates Fe (II) and lipid peroxidation. | Cell Death Discovery

Fig. 2: The 25-HC regulates Fe (II) and lipid peroxidation.

From: Oxysterol 25-hydroxycholesterol activation of ferritinophagy inhibits the development of squamous intraepithelial lesion of cervix in HPV-positive patients

Fig. 2

A Concentration of 25-HC in H8 cells, SiHa cells, and HeLa cells was detected with an Elisa Assay Kit. All data are from three independent experiments. The data are presented as the mean ± SD values (n ≥ 3). B The H8 cells, SiHa cells, and HeLa cells were treated with 25-HC at indicated concentration for 24 h. The cell viability was detected with a CCK8 biochemical detection assay (n = 3). C, D The H8 cells, SiHa cells and HeLa cells were treated with 25-HC at indicated concentration for 24 h. Then they were fixed and stained with FerroOrange (red) to identify Fe (II). Scale bar, 100 μm. E The H8 cells, SiHa cells and HeLa cells were treated with 25-HC at indicated concentration for 24 h. Then they were fixed and stained with BDP 581/591 C11 to identify oxidized state of lipid peroxide (green) and reduced state of lipid peroxide (red). Scale bar, 100 μm. All data are from three independent experiments. The data are presented as the mean ± SD values (n ≥ 3). *P < 0.05; **P < 0.01, ***P < 0.001, ns: not significant.

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