Fig. 5: Long-term exposure to 25-HC enhances lipid peroxidation. | Cell Death Discovery

Fig. 5: Long-term exposure to 25-HC enhances lipid peroxidation.

From: Oxysterol 25-hydroxycholesterol activation of ferritinophagy inhibits the development of squamous intraepithelial lesion of cervix in HPV-positive patients

Fig. 5

A The H8 cells and SiHa cells were treated with 25-HC for 9 days (long-term). Then they were fixed and stained with FerroOrange (red) to identify Fe (II). Scale bar, 50 μm. B The H8 cells and SiHa cells were stained with BDP 581/591 C11 to identify oxidized state of lipid peroxide (green) and reduced state of lipid peroxide (red). Scale bar, 100 μm. C The H8 cells and SiHa cells were treated with 25-HC for 24 h (short-term) or 9 days (long-term). Lysates of the H8 cells and SiHa cells were immunoblotted for GPX4, KRAS, and ACTB (n = 3). The data are normalized to the ACTB control. All data are from three independent experiments. The data are presented as the mean ± SD values (n ≥ 3). *P < 0.05.

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