Fig. 6: 25-HC is associated with SIL development and CSCC. | Cell Death Discovery

Fig. 6: 25-HC is associated with SIL development and CSCC.

From: Oxysterol 25-hydroxycholesterol activation of ferritinophagy inhibits the development of squamous intraepithelial lesion of cervix in HPV-positive patients

Fig. 6

A Concentration of 25-HC in LSIL, HSIL, and CSCC tissues was detected with an Elisa Assay Kit. B Survival probability analysis of the expression of CH25H in patients with CSCC using Kaplan–Meier Plotter. Black line indicates low expression and red line indicates high expression. C qPCR was used to analyze the mRNA levels of CH25H in LSIL, HSIL, and CSCC tissues. D Tissue sections in LSIL, HSIL and CSCC were immunostained with anti-CH25H antibody using IHC. Scale bar, 200 μm. E qPCR was used to analyze the mRNA levels of NCOA4 in LSIL, HSIL, and CSCC tissues. F Tissue sections in LSIL, HSIL, and CSCC were immunostained with anti- NCOA4 antibody using IHC. Scale bar, 200 μm. G qPCR was used to analyze the mRNA levels of FTH1 in LSIL, HSIL, and CSCC tissues. H Tissue sections in LSIL, HSIL, and CSCC were immunostained with anti-FTH1 antibody using IHC. Scale bar, 200 μm. All data are from three independent experiments. The data are presented as the mean ± SD values (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant.

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