Fig. 2: Effects of PL on the colony formation of human and mouse AML cell lines and normal primary bone marrow cells. | Cell Death Discovery

Fig. 2: Effects of PL on the colony formation of human and mouse AML cell lines and normal primary bone marrow cells.

From: Piplartine eliminates CD34 + AML stem/progenitor cells by inducing oxidative stress and suppressing NF-κB signalling

Fig. 2

Colony-forming ability of (A, B) MV4-11, (C, D) MOLM-13, (E, F) OCI-AML-3 and (G, H) Cas9 FLT3-ITD MLL-AF4 and (G, I) MLL-AF9 cells after treatment with PL at different concentrations and representative colony formation microscopy images. J Representative colony formation microscopy images of primary mouse bone marrow cells. K, L Effects of PL on the total number of colonies and the number of different colony types derived from primary mouse bone marrow cells. Colony-forming cell: M, monocyte; G, granulocyte; GM, granulocyte/macrophage; GEM, granulocyte, erythrocyte, and macrophage. The colony type is indicated by red arrows. The vehicle (0.2% DMSO) was used as a negative control (CTL). Scale bar: 1000 μm. The data are shown as the mean ± S.E.M. of three independent experiments carried out in duplicate. * p < 0.05 compared to CTL by one-way ANOVA followed by Dunnett’s multiple comparisons test.

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