Fig. 1: MCT4 is highly expressed in Cancer-Associated Fibroblasts. | Cell Death Discovery

Fig. 1: MCT4 is highly expressed in Cancer-Associated Fibroblasts.

From: MCT4-driven CAF-mediated metabolic reprogramming in breast cancer microenvironment is a vulnerability targetable by miR-425-5p

Fig. 1

Representative Western blot analysis of MCT4 performed on plasmamembrane fractions of Cancer-Associated Fibroblasts (CAFs) (n = 4) and Normal Fibroblasts (NFs) (n = 3). Na + /K+ ATPase was used for data normalization of the fractionation in the sample preparation. Densitometry data are expressed after normalization on Na + /K+ ATPase band intensity A. A representative confocal microscopy image of MCT4 expression in CAFs (n = 3) (DAPI blue, MCT4 red) B. Three different subpopulations of two primary fibroblasts were collected at different growing distances from the tumor (intratumoral, IT; peritumoral, PT; normal, NF) and MCT4 gene expression was evaluated by quantitative real-time PCR. Values are expressed as a mean of two biological replicates of technical duplicates C. Culture media from NFs (n = 2) and CAFs (n = 2) were collected and assessed by a colorimetric L-lactate assay. Values are expressed as mean of two technical replicates D. Graph shows mean ± SD. * P values < 0.05; ***p < 0.001. Bar = 100 μm.

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