Fig. 3: miR425-5p-induced metabolic changes in Cancer-Associated Fibroblasts. | Cell Death Discovery

Fig. 3: miR425-5p-induced metabolic changes in Cancer-Associated Fibroblasts.

From: MCT4-driven CAF-mediated metabolic reprogramming in breast cancer microenvironment is a vulnerability targetable by miR-425-5p

Fig. 3

L-lactate assessed with a colorimetric assay in Cancer-Associated Fibroblasts (CAFs n = 2) and culture media 72 h after miR-425-5p transfection. Values are expressed as a mean of two biological replicates of technical duplicates A. L-lactate assessed in Normal Fibroblasts (NFs n = 2) and culture media 48 h after antimiR-425-5p transfection. Values are expressed as a mean of two biological replicates of technical duplicates B. Representative Western blot C and real-time analysis D of GLUT-1 expression in CAFs (n = 2) upon miR-425-5p transfection. Data are expressed as mean of two technical replicates. Metabolic changes in CAFs transfected with miR-425-5p, as assessed by glycolytic proton efflux rate (glycoPER) kinetics E, extracellular acidification rate (ECAR) F, proton efflux rate (PER) G, and ATP production H. Data are presented as a mean of two biological replicates of eight technical replicates. Data are mean percentage ± SD over control. * P values < 0.05; ** P values < 0.01.

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