Fig. 5: RIPK1 inhibitor prevents vascular endothelial cell dysfunction induced by activated neutrophils. | Cell Death Discovery

Fig. 5: RIPK1 inhibitor prevents vascular endothelial cell dysfunction induced by activated neutrophils.

From: RIPK1 inhibitor ameliorates pulmonary injury by modulating the function of neutrophils and vascular endothelial cells

Fig. 5

A The flow diagram of neutrophil-vascular endothelial cell co-cultured system. Neutrophils were isolated from mouse bone marrow and incubated with 1 μg/mL LPS. After 3 h, the supernatant of neutrophils and GSK2982772 (20 μM, 10 μM) were added to the bEnd.3 cells for 24 h. Gene expressions of cytokines (B), chemokines (C, D) and adhesion molecules (E) were determined in co-cultured bEnd.3 cells. F Representative Western blot and statistical analysis of ICAM-1 and VCAM-1 in co-cultured bEnd.3 cells. These results are representative of three independent experiments. All data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared to Sup. Neu group. #P < 0.05, ##P < 0.01, ###P < 0.001 compared to blank group.

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