Fig. 1: NAP1L1 promotes HCC cell proliferation and inhibits apoptosis. | Cell Death Discovery

Fig. 1: NAP1L1 promotes HCC cell proliferation and inhibits apoptosis.

From: NAP1L1 regulates BIRC2 ubiquitination modification via E3 ubiquitin ligase UBR4 and hence determines hepatocellular carcinoma progression

Fig. 1

A, B CCK-8 assay was used to assess the proliferation of Huh7 and LM3 cells after knockdown or overexpression of NAP1L1. C, D Colony formation assay was performed to assess the ability of Huh7 and LM3 cells to form clones after knockdown or overexpression of NAP1L1. E, F Flow cytometry was used to assess the apoptosis levels of Huh7 and LM3 cells after knockdown or overexpression of NAP1L1. G Transmission electron microscopy was used to observe the morphological and apoptotic features of Huh7 and LM3 cells after knockdown of NAP1L1 (red arrows indicate apoptotic bodies; ×2.5k visual field, scale bar = 5 μm; ×8.0k, scale bar = 1 μm). H Laser confocal microscopy was used to assess the mitochondrial membrane potential of Huh7 and LM3 cells after knockdown of NAP1L1. Red fluorescence indicates normal mitochondrial membrane potential, whereas green fluorescence indicates depolarised mitochondrial membrane potential (×40 visual field, scale bar = 50 μm). Data are representative of three independent experiments and are expressed as the mean ± SD (*p < 0.05 versus control; **p < 0.01; ***p < 0.001).

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