Fig. 6: Overexpression of BIRC2 promotes HCC cell proliferation and reverses apoptosis induced by knockdown of NAP1L1. | Cell Death Discovery

Fig. 6: Overexpression of BIRC2 promotes HCC cell proliferation and reverses apoptosis induced by knockdown of NAP1L1.

From: NAP1L1 regulates BIRC2 ubiquitination modification via E3 ubiquitin ligase UBR4 and hence determines hepatocellular carcinoma progression

Fig. 6

A, B CCK-8 assay was performed to assess the effects of BIRC2 overexpression on the proliferation of Huh7 and LM3 cells with NAP1L1 knockdown. C Colony formation assay was performed to assess the effects of BIRC2 overexpression on the colony-forming ability of Huh7 and LM3 cells with NAP1L1 knockdown. D Western blotting was performed to assess the effects of BIRC2 overexpression on the expression of apoptotic proteins in Huh7/LM3 cells with NAP1L1 knockdown. E, F Flow cytometry was performed to assess the effects of BIRC2 overexpression on the apoptosis levels of Huh7/LM3 cells with NAP1L1 knockdown. G Transmission electron microscopy was performed to assess the effects of BIRC2 overexpression on the morphological and apoptotic features of Huh7/LM3 cells with NAP1L1 knockdown. Data are representative of three independent experiments and are expressed as the mean ± SD (*p < 0.05 versus control; **p < 0.01; ***p < 0.001).

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