Fig. 6: CKS1 induces the release of immunogenic molecules.

A, B Cells were treated with 20 µM or 100 µM CKS1 for 30 min and the amount of ATP released in the culture media was quantified. Means ± SEM, N = 3, Dunnet’s test (*p < 0.05, ***p < 0.005). C, D Cells were treated with 100 µM CKS1 for 6 h and the amount of HMGB-1 released in the culture media was quantified. Means ± SEM, N = 3, Welch’s t-test (*p < 0.05). E DC2.4 cells were treated with either complete media (control), conditioned media from 4T1 cells, 100 µM CKS1, or conditioned media from 4T1 cells treated with 100 µM CKS1. The relative expression level normalized to the expression level in the control condition is shown. Means ± SEM, N = 4, Dunnet’s test (*p < 0.05, **p < 0.01).