Fig. 2: NREP is transcriptional activated by HIF-1α. | Cell Death Discovery

Fig. 2: NREP is transcriptional activated by HIF-1α.

From: NREP, transcriptionally upregulated by HIF-1α, aggravates breast cancer cell growth and metastasis by promoting glycolysis

Fig. 2

A The expression of NREP in hypoxia and normoxia BC cells (data from GSE3188 and GSE111259). B The expression of NREP in HIF-1α depleted BC cells (data from GSE3188). C The mRNA expression of NREP in different BC cell lines (SK-BR-3, MCF-7, MDA-MB-468, MDA-MB-231) at normoxia or hypoxia was measured by qPCR. The mRNA expression of HIF-1α (D) and NREP (E) in HIF-1α inhibited MDA-MB-468 or MDA-MB-231 cells were measured by qPCR. F The protein expression and quantification data of NREP and HIF-1α in MDA-MB-468 or MDA-MB-231 cells were measured by western blot. G, H Luciferase reporter assay was used to confirm the binding sites of HIF-1α on NREP promoter. I, J ChIP-PCR assay was used to confirm that HIF-1α could directly bind with the promoter of NREP. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01.

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