Fig. 3: NREP promotes BC cell proliferation and angiogenesis in vitro. | Cell Death Discovery

Fig. 3: NREP promotes BC cell proliferation and angiogenesis in vitro.

From: NREP, transcriptionally upregulated by HIF-1α, aggravates breast cancer cell growth and metastasis by promoting glycolysis

Fig. 3

NREP was down-/up-regulated in MDA-MB-468 and MDA-MB-231 cells. A, B The mRNA expression of NREP in MDA-MB-468 or MDA-MB-231 cells was measured by qPCR. C, D The protein expression of NREP in MDA-MB-468 or MDA-MB-231 cells was measured by western blot. E, F Cell proliferation was analyzed by MTT assay in MDA-MB-468 or MDA-MB-231 cells. G, H The cell cycle was analyzed by flow cytometry in MDA-MB-468 or MDA-MB-231 cells. I, J Colony formation images and quantification data in MDA-MB-468 or MDA-MB-231 cells. K, L EdU staining images (green light) of MDA-MB-468 cells or MDA-MB-231 cells. DAPI: blue. M Cell apoptosis was analyzed by flow cytometry in MDA-MB-468 or MDA-MB-231 cells. N Caspase-3 activity in MDA-MB-468 or MDA-MB-231 cells. O, P Tube formation images and quantification data of HUVECs in tumor-conditioned medium. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01.

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