Fig. 4: NREP promotes BC cell migration, invasion, and metastasis. | Cell Death Discovery

Fig. 4: NREP promotes BC cell migration, invasion, and metastasis.

From: NREP, transcriptionally upregulated by HIF-1α, aggravates breast cancer cell growth and metastasis by promoting glycolysis

Fig. 4

A, B Cell migration was measured by transwell assay in NREP down-/up-regulated MDA-MB-231 cells. C, D Cell invasion was measured by transwell assay in NREP down-/up-regulated MDA-MB-231 cells. E The morphological changes of MDA-MB-231 cells were observed under a light microscope. F The protein expression and quantification data of E-cadherin, N-cadherin, SLUG, and MMP9 in NREP down-/up-regulated MDA-MB-231 cells were analyzed by western blot. G, H The mRNA expression of E-cadherin and N-cadherin in NREP down-/up-regulated MDA-MB-231 cells was analyzed by qPCR. I The images of metastasis lesions in mouse lung tissues. J, K H&E staining images and quantification data of metastasis lesions in mouse lung tissues. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01.

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