Fig. 5: MT suppresses LPS-induced ferroptosis in RAW264.7 cells via MT1.
From: Melatonin alleviates septic ARDS by inhibiting NCOA4-mediated ferritinophagy in alveolar macrophages
A The mRNA expression levels of MT1 after knockdown of MT1 receptor in RAW264.7 cells with siRNA. Intracellular GSH (B), MDA (C), and Fe2+ (D) contents were detected by corresponding assay kits. E–G The FTH1 and GPX4 protein levels were detected by western blot. Lipid peroxidation levels detected by the C11 BODIPY assay kit were observed under a fluorescence microscope (H) and statistics as shown in (I). Cell proliferation detected by the EdU assay kit was observed under a fluorescence microscope (J) and statistics as shown in (K). Data are expressed as mean ± SD (n = 6), *p < 0.05, **p < 0.01 indicate significant differences from each group, NS indicates no significance.
