Fig. 6: MT alleviates LPS-induced ferroptosis in RAW264.7 cells by inhibiting ferritinophagy.
From: Melatonin alleviates septic ARDS by inhibiting NCOA4-mediated ferritinophagy in alveolar macrophages
Intracellular MDA (A), and Fe2+ (B) contents were detected by corresponding assay kits. Lipid peroxidation levels detected by the C11 BODIPY assay kit were observed under a fluorescence microscope (I) and statistics as shown in (C). Cell proliferation detected by the EdU assay kit was observed under a fluorescence microscope (J) and statistics as shown in (D). E–H The FTH1, GPX4 and LC3 protein levels were detected by western blotting. Data are expressed as mean ± SD (n = 6), *p < 0.05, **p < 0.01 indicate significant differences from each group, NS indicates no significance.
