Fig. 7: MT protects RAW264.7 cells from LPS-induced ferroptosis by inhibiting NCOA4-mediated ferritinophagy.
From: Melatonin alleviates septic ARDS by inhibiting NCOA4-mediated ferritinophagy in alveolar macrophages
A The transfection efficiency of lentivirus was detected by qPCR. Intracellular GSH (B), MDA (C), and Fe2+ (D) contents were detected by corresponding assay kits. E–H The FTH1, GPX4 and NCOA4 protein levels were detected by western blotting. Lipid peroxidation levels detected by the C11 BODIPY assay kit were observed under a fluorescence microscope (I) and statistics as shown in (J). Cell proliferation detected by the EdU assay kit was observed under a fluorescence microscope (K) and statistics as shown in (L). Mitochondrial membrane potential changes detected by JC-1 mitochondrial assay kit was observed (M) under fluorescence microscope and statistics as shown in (N). O Mitochondrial morphological changes under electron microscopy (The arrows mark mitochondria). Data are expressed as mean ± SD (n = 6), *p < 0.05, **p < 0.01 indicate significant differences from each group, NS indicates no significance.
