Fig. 3: Overexpression of IL-17 activates LX2 cell line, promotes their proliferation and migration, and inhibits their apoptosis. | Cell Death Discovery

Fig. 3: Overexpression of IL-17 activates LX2 cell line, promotes their proliferation and migration, and inhibits their apoptosis.

From: IL-17a promotes hepatocellular carcinoma by increasing FAP expression in hepatic stellate cells via activation of the STAT3 signaling pathway

Fig. 3

A mRNA expression and protein expression levels of IL-17 in the IL-17-overexpressing LX2 cell line (IL-17-OE), the corresponding control cell line (IL-17-NC), and the untreated control cell line (LX2 MOCK). B Representative images and statistical analysis of the proliferation of IL-17-OE and IL-17-NC cells by a plate colony formation assay. C Representative images of the proliferation of IL-17-OE and IL-17-NC cells in the EdU incorporation assay (immunofluorescence staining, 200x, blue fluorescence, nuclei; red fluorescence, proliferating cells). D Proliferation rate of IL-17-OE and IL-17-NC cells in the EdU incorporation assay. E Proliferation curves of IL-17-OE and IL-17-NC LX2 cells as determined by a CCK8 assay. F Representative images and statistical analysis of the migration of IL-17-OE and IL-17-NC cells in the Transwell assay. G Representative images of the migration of IL-17-OE and IL-17-NC cells in the wound healing assay. H Statistical analysis of wound closure by IL-17-OE and IL-17-NC cells in the wound healing assay. I Representative images of apoptosis in IL-17-OE and IL-17-NC cells in the TUNEL assay (immunofluorescence staining, 200×, blue fluorescence, nuclei; green fluorescence, nuclei in cells undergoing apoptosis). J Apoptosis rates of IL-17-OE and IL-17-NC cells in the TUNEL assay. K mRNA expression of several hepatic stellate cell-related genes in LX2 cells after IL-17 overexpression. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

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