Fig. 4: Overexpression of FAP activates LX2 cell line, promotes their proliferation and migration, and inhibits their apoptosis. | Cell Death Discovery

Fig. 4: Overexpression of FAP activates LX2 cell line, promotes their proliferation and migration, and inhibits their apoptosis.

From: IL-17a promotes hepatocellular carcinoma by increasing FAP expression in hepatic stellate cells via activation of the STAT3 signaling pathway

Fig. 4

A mRNA expression and protein expression of FAP in the FAP overexpression LX2 cell line (FAP-OE), the corresponding control cell line (FAP-NC) and the untreated control cell line (LX2 MOCK). B Representative images and statistical analysis of the proliferation of FAP-OE and FAP-NC cells in the plate colony formation assay. C Representative images of the proliferation of FAP-OE and FAP-NC cells in the EdU incorporation assay (immunofluorescence staining, 200x, blue fluorescence, nuclei; red fluorescence, proliferating cells). D Proliferation rates of FAP-OE and FAP-NC cells in the EdU incorporation assay. E Proliferation curves of FAP-OE and FAP-NC LX2 cells as determined by a CCK8 assay. F Representative images and statistical analysis of the migration of FAP-OE and FAP-NC cells in the Transwell assay. G Representative images of the migration of FAP-OE and FAP-NC cells in the wound healing assay. H Statistical analysis of wound closure by FAP-OE and FAP-NC cells in the wound healing assay. I Representative images of apoptosis in FAP-OE and FAP-NC cell lines in the TUNEL assay (immunofluorescence staining, 200x, blue fluorescence, nuclei; green fluorescence, nuclei in cells undergoing apoptosis). J Apoptosis rates of FAP-OE and FAP-NC cells in the TUNEL assay. K mRNA expression of several hepatic stellate cell-related genes in LX2 cells after FAP overexpression. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

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