Fig. 6: Characterization of KCNQ1OT1-sgRNA-Huh7 cells compared to Cas9-Huh7 cells. | Cell Death Discovery

Fig. 6: Characterization of KCNQ1OT1-sgRNA-Huh7 cells compared to Cas9-Huh7 cells.

From: Sponging of five tumour suppressor miRNAs by lncRNA-KCNQ1OT1 activates BMPR1A/BMPR1B-ACVR2A/ACVR2B signalling and promotes chemoresistance in hepatocellular carcinoma

Fig. 6

(A) Immunoblot analysis of the downstream BMP signalling genes SMAD5, p-SMAD5, ERK1/2, p-ERK1/2 was performed with the lysates of Cas9-Huh7 and KCNQ1OT1-sgRNA-Huh7 cells. qRT-PCR and Immunoblot analysis of (B) EMT markers and (C) stemness markers was done with Cas9-Huh7 and KCNQ1OT1-sgRNA-Huh7 cells. The effect of KCNQ1OT1 deletion on cancer progression was measured by quantifying (D) spheroid number, (E) chemosensitivity to doxorubicin, (F) proliferation, (G) migration, and (H) invasion properties of Cas9-Huh7 and KCNQ1OT1-sgRNA-Huh7 cells. p value was calculated using unpaired student’s t-test for B–H. *** indicates p value < 0.001.

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