Fig. 6: ATG7 was essential for SNHG1-mediated sunitinib resistance and autophagy in RCC cells.

RCC cell resistant strains were transfected with sh-SNHG1, pcDNA3.1-ATG7 and negative controls. A RT-qPCR was used to test ATG7 expression in RCC cell resistant strains with sh-SNHG1 and pcDNA3.1-ATG7. B Flow cytometric detection of cell apoptosis in different groups. C The fluorescence intensity of GFP-LC3 autophagosomes was detected in different groups. Scale bar, 25 μm. D The protein levels of autophagy-related molecules were assessed by western blot in different groups. All data are represented as the mean ± SD. Experiments were repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001.