Fig. 7: SNHG1 exerted an oncogenic role by increasing ATG7 expression through binding to PTBP1. | Cell Death Discovery

Fig. 7: SNHG1 exerted an oncogenic role by increasing ATG7 expression through binding to PTBP1.

From: An oncogenic role of lncRNA SNHG1 promotes ATG7 expression and autophagy involving tumor progression and sunitinib resistance of Renal Cell Carcinoma

Fig. 7

A Subcellular fractionation assay was used to detect the expression and location of SNHG1 in RCC cell resistant strains. B, C RNA pull-down and RIP assays verified the binding relationship between SNHG1 and PTBP1 in RCC cell resistant strains. D RT-qPCR was performed to detect ATG7 expression in RCC cell resistant strains with sh-NC or sh-SNHG1. E RT-qPCR was conducted to measure ATG7 expression in RCC cell resistant strains with pcDNA3.1-SNHG1, sh-PTBP1 and negative controls. F, G RIP was utilized to test the effect of SNHG1 on the recruitment of PTBP1 to ATG7 mRNA in RCC cell resistant strains. H, I RT-qPCR analysis of the half-life of ATG7 mRNA in RCC cell resistant strains with pcDNA3.1-SNHG1, sh-PTBP1 and negative controls. All data are represented as mean ± SD. Experiments were repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001.

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