Fig. 2: LCA decreases EMT marker expression and the invasion ability of pancreatic adenocarcinoma cells.
From: The bacterial metabolite, lithocholic acid, has antineoplastic effects in pancreatic adenocarcinoma
A Capan-2 and BxPC-3 cells were treated with LCA (0.03 µM) or DMSO for 48 h, and EMT marker (mesenchymal: β-catenin, Snail; epithelial: ZO1) and Claudin-1 protein expression levels were determined (n = 3, upper panels: representative western blots, lower panels: densitometric analyses of western blots). B Cell invasion was measured after treating Capan-2 cells with 0.03 µM LCA for 48 h. The invasion index was calculated (n = 3). Means ± SEM are shown. * and **; p < 0.05 and p < 0.01, respectively, DMSO-treated vs. LCA-treated groups. DMSO dimethyl sulfoxide, LCA lithocholic acid, ZO1 zona occludens 1.
