Fig. 1: PKCδ was elevated in high glucose-treated HUVECs and the skin of type 2 diabetes mice. | Cell Death Discovery

Fig. 1: PKCδ was elevated in high glucose-treated HUVECs and the skin of type 2 diabetes mice.

From: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

Fig. 1

A mRNA level of PKCδ was determined using qPCR after treatment with high glucose (22.5 mM) for 48 h respectively. B, C Protein expression detection with western blot and quantification of PKCδ after HG treatment for 72 h. D, E PKCδ detection by immunofluorescence and quantification of fluorescence intensity after HG treatment for 24 h. F mRNA level of PKCδ in skin tissues of STZ-induced diabetes mice. G, H Protein level and quantification of PKCδ in skin tissues of STZ-induced diabetes mice. I mRNA expression of PKCδ in skin tissues isolated from db/db mice. J, K Protein expression and quantification of PKCδ in skin tissues isolated from db/db mice. Each experiment was replicated at least thrice, and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, *p < 0.05, **p < 0.01 vs controls.

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