Fig. 2: PKCδ knockdown by siRNA ameliorated high glucose-induced HUVECs apoptosis. | Cell Death Discovery

Fig. 2: PKCδ knockdown by siRNA ameliorated high glucose-induced HUVECs apoptosis.

From: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

Fig. 2

A qPCR analysis of mRNA level of PKCδ after si-PKCδ transfection for 48 h. B, C Western blot and quantification of PKCδ protein level after transfection for 72 h. D, E The protein level of pro-caspase 3 and cleaved-caspase 3 and quantification of cleaved-caspase 3 72 h after transfection. F, G TUNEL assay and quantification of TUNEL-positive cells 72 h after transfection. H, I Flow cytometry assay and quantification of apoptotic cells 72 h after transfection. Each experiment was replicated for at least thrice and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, *p < 0.05, **p < 0.01 vs controls.

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