Fig. 5: The expression of HMGCS1, HMGCR and the concentration of free cholesterol were upregulated in high glucose-induced HUVECs. | Cell Death Discovery

Fig. 5: The expression of HMGCS1, HMGCR and the concentration of free cholesterol were upregulated in high glucose-induced HUVECs.

From: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

Fig. 5

A, B qPCR analysis of the mRNA level of HMGCS1 and HMGCR after high glucose treatment for 48 h. C–F Western blot and quantification of the protein level of HMGCS1 and HMGCR after high glucose treatment for 72 h. G–J Immunofluorescence of HMGCS1 and HMGCR and quantification of fluorescence intensity after high glucose treatment for 72 h. K Free cholesterol assay 72 h after high glucose treatment. Each experiment was replicated at least thrice, and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ****p < 0.0001 vs controls.

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