Fig. 6: PKCδ knockdown by siRNA decreased HMGCS1, HMGCR expression and free cholesterol level in high glucose-treated HUVECs. | Cell Death Discovery

Fig. 6: PKCδ knockdown by siRNA decreased HMGCS1, HMGCR expression and free cholesterol level in high glucose-treated HUVECs.

From: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

Fig. 6

A, B qPCR analysis of the mRNA level of HMGCS1 and HMGCR after transfection for 72 h. C–F Western blot and quantification of the protein level of HMGCS1 and HMGCR 72 h after transfection. G–J Immunofluorescence of HMGCS1 and HMGCR and quantification of fluorescence intensity after transfection for 72 h. K Free cholesterol assay 72 h after transfection. Each experiment was replicated at least thrice and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 vs controls.

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