Fig. 7: Cholesterol restored high glucose-induced apoptosis in si-PKCδ-treated HUVECs. | Cell Death Discovery

Fig. 7: Cholesterol restored high glucose-induced apoptosis in si-PKCδ-treated HUVECs.

From: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

Fig. 7

A, B Western blot and quantification of cleaved-caspase 3 after cholesterol treatment for 24 h in si-PKCδ-transfected HUVECs. C, D TUNEL assay and quantification of TUNEL-positive cells after cholesterol treatment for 24 h in si-PKCδ-transfected HUVECs. E, F Flow cytometry assay and quantification of apoptotic cells after cholesterol treatment for 24 h in si-PKCδ-transfected HUVECs. Each experiment was replicated at least thrice and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, **p < 0.01, ***p < 0.001, vs controls.

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