Fig. 2: Immunofluorescence cytochemistry (IFC) and Senescence-associated-β-galactosidase(SAβG) staining were used to localize p21, β-actin and β-glacotosidase in ESC before (Control) and after IL-1β stimulation. | Cell Death Discovery

Fig. 2: Immunofluorescence cytochemistry (IFC) and Senescence-associated-β-galactosidase(SAβG) staining were used to localize p21, β-actin and β-glacotosidase in ESC before (Control) and after IL-1β stimulation.

From: Interleukin-1β induces and accelerates human endometrial stromal cell senescence and impairs decidualization via the c-Jun N-terminal kinase pathway

Fig. 2

A P3 ESC show increased nuclear p21 (green signal, left panel) after IL-1β-treatment. β-Actin (red) staining is more diffuse in IL-1β-treated ESC and the cellular orientation is more parallel. Merged channel images (yellow signal, right panels) are included. Magnification × 200. B SA-β-gal staining was used to detect β-galactosidase-positive signals. There was an intense blue color in the cytoplasm of ESC following 24 h of IL-1β treatment (right panel) compared to the untreated control (left panel). Magnification × 200.

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