Fig. 3: Upregulation of SASP protein biomarkers in response to IL-1β by western blot analysis. | Cell Death Discovery

Fig. 3: Upregulation of SASP protein biomarkers in response to IL-1β by western blot analysis.

From: Interleukin-1β induces and accelerates human endometrial stromal cell senescence and impairs decidualization via the c-Jun N-terminal kinase pathway

Fig. 3

A After 48 h incubation, Western blotting shows that IL-1β increased IL-1β, IL-6, TNF-α, MMP3, p16, p21, CCL2 and phospho-histone H2A.X in P3 ESC. B Time-course experiments reveal that IL-1β upregulated IL-1β, IL-6, TNF-α, MMP3, p16, p21, HMGB1, CCL2, CCL5 and phospho-histone H2A.X at 24, 48 and 72 h (lanes 2, 4 and 6). Co-incubation in the presence of the JNK inhibitor SP blocked IL-1β-mediated upregulation at all time points (lanes 3, 5 and 7). Incubation with SP alone (lane 8) suppressed many biomarkers below basal (Control, lane 1) levels. β-Actin levels were not affected. Molecular masses (kDa) are indicated by arrows at right. C Based on the time course experiments, the three key senescence-associated biomarkers indicated that the baseline levels of IL-1β and IL-6 were notably low and undetectable by the western blot method at all three time points. Meanwhile, MMP3 expression levels in the untreated control group remained consistently similar across the three observed time points. β-Actin levels were not affected. Molecular masses (kDa) are indicated by arrows at right.

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