Fig. 3: Nicotine promoted the metastasis of LSCC by activating CHRNA5 in vitro. | Cell Death Discovery

Fig. 3: Nicotine promoted the metastasis of LSCC by activating CHRNA5 in vitro.

From: Nicotine-induced activation of cholinergic receptor nicotinic alpha 5 subunit mediates the malignant behaviours of laryngeal squamous epithelial cells by interacting with RABL6

Fig. 3

qRT-PCR (A) and western blotting (B) were performed to examine the mRNA and protein expression of CHRNA5, respectively, in AMC-HN8 and FD-LSC-1 cells infected with sh‐control, sh‐CHRNA5-1, sh‐CHRNA5-2 and sh‐CHRNA5-3 lentiviruses. Wound healing assay (C, D, Scale bar: 500 μm) and transwell assay (E, F, Scale bar: 100 μm) demonstrated that CHRNA5 knockdown significantly inhibited the migratory and invasive capabilities of LSCC cells; however, these effects were reversed by nicotine. Representative images (G) and quantitative analysis (H) of cell adhesion assay, which demonstrated that CHRNA5 knockdown effectively inhibited the adhesion of LSCC cells to the extracellular matrix; however, these results were reversed by nicotine.

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