Fig. 4: Nicotine promoted the metastasis of LSCC by activating CHRNA5 in vivo. | Cell Death Discovery

Fig. 4: Nicotine promoted the metastasis of LSCC by activating CHRNA5 in vivo.

From: Nicotine-induced activation of cholinergic receptor nicotinic alpha 5 subunit mediates the malignant behaviours of laryngeal squamous epithelial cells by interacting with RABL6

Fig. 4

Luciferase-labelled AMC-HN8 cells stably expressing sh-CHRNA5 and sh-NC were injected into the tail veins and footpad of mice to construct tail vein metastasis and inguinal lymph node metastasis models, respectively. A Representative bioluminescence images of the lungs of nude mice treated with sh-NC, sh-CHRNA5 and sh-CHRNA5 after nicotine stimulation. B Representative HE staining of the lung tissue sections of mice. Scale bar: 100 μm. C Histogram analysis of the number of pulmonary metastatic nodules. D Representative bioluminescence images of the right lower limbs of nude mice treated with sh-NC, sh-CHRNA5 and sh-CHRNA5 after nicotine stimulation. E Representative images of the gross appearance of inguinal lymph nodes in nude mice. Measured lymph node volumes (F) and weights (G). H Metastatic ratio of inguinal lymph nodes. I Representative images of HE and IHC staining of inguinal lymph nodes with pan-cytokeratin. Scale bar: 20 μm.

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