Fig. 6: Nicotine-induced activation of CHRNA5 promoted RABL6 expression to facilitate the metastasis of LSCC. | Cell Death Discovery

Fig. 6: Nicotine-induced activation of CHRNA5 promoted RABL6 expression to facilitate the metastasis of LSCC.

From: Nicotine-induced activation of cholinergic receptor nicotinic alpha 5 subunit mediates the malignant behaviours of laryngeal squamous epithelial cells by interacting with RABL6

Fig. 6

A The immunoprecipitated proteins were separated via SDS-PAGE and stained with Coomassie brilliant blue. B Relative abundance of peptidases identified via mass spectrometry. C Statistical analysis of the results of mass spectrometry. Wound healing (D, E, Scale bar: 500 μm) and transwell (F, G, Scale bar: 100 μm) assays demonstrated that RABL6 overexpression significantly promoted the migratory and invasive capabilities of LSCC cells; however, CHRNA5 knockdown reversed these effects. H Western blotting was performed to evaluate RABL6 expression in LSCC cells infected with sh-NC or sh-CHRNA5 and cultured in the presence or absence of nicotine. I Differential expression analysis of RABL6 in TCGA cohort. J Differential expression analysis of RABL6 in patients with LSCC stratified based on the smoking status in TCGA cohort. K Correlation between CHRNA5 and RABL6 expression in TCGA cohort.

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