Fig. 4: Plck-GAPDH tumor transplanted in NSG mice has the same immune and metabolic phenotype as the primary mAITL lymphoma. | Cell Death Discovery

Fig. 4: Plck-GAPDH tumor transplanted in NSG mice has the same immune and metabolic phenotype as the primary mAITL lymphoma.

From: Dependence on mitochondrial respiration of malignant T cells reveals a new therapeutic target for angioimmunoblastic T-cell lymphoma

Fig. 4

Total splenocytes from tumor-bearing Plck-GAPDH mice were transferred to NSG mice, which were sacrificed 8–12 weeks post injection. A The % CD4+ DP-1high T cells gated on total CD4+ T cells and GC B cells (CD95 + GL-7+ gated on B220 + CD19+ cells) in spleens from donor plck-GAPDH mice and NSG recipient mice after 8–12 weeks engraftment were analyzed by FACS and summarized in a histogram (mean ± SD; n = 3, ns = not significant). B FACS analysis of CD8+ and CD4+ T cells of plck-GAPDH donor mice and NSG recipient mice, stained for mitochondrial content by Mitotracker green (MTG) and summarized in histogram (mean ± SD; n = 3, ns = not significant). C Analysis of the metabolic dependance on glucose or mitochondria for the CD4+ T cells in the spleen of of plck-GAPDH donor mice and NSG recipient mice at sacrifice by SENITH metabolic analysis (mean ± SD; n = 3, ns = not significant).

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