Fig. 3: CXCL12 is downregulated in ICC samples with LNM and enhances migration of DCs. | Cell Death Discovery

Fig. 3: CXCL12 is downregulated in ICC samples with LNM and enhances migration of DCs.

From: Mobilization and activation of tumor-infiltrating dendritic cells inhibits lymph node metastasis in intrahepatic cholangiocarcinoma

Fig. 3

A Venn diagram displaying the chemokines significantly downregulated in human ICC samples with LNM in FU-iCCA cohort. B Scatter plots showing the normalized transcript levels of CXCL12 in ICC samples with or without LNM in FU-iCCA cohort, as well as in adjacent normal liver tissues from datasets GSE107943 and GSE76927 (C). D Survival analysis of ICC patients grouped by mRNA levels of CXCL12 in FU-iCCA cohort. E Spearman correlation analysis of CXCL12 mRNA expression with conventional DC marker genes CLEC9A and CLEC10A, as well as CD8A transcripts in FU-iCCA cohort. F Representative IHC images of CXCL12 staining density and quantification by H-score in ICC samples with or without LNM, as well as in adjacent non-tumorous liver tissues from TMA cohort. G Spearman correlation of CXCL12 staining density with cell counts of CD45+ CD11c+ DCs in TMA cohort. H Kaplan–Meier survival curves for OS of 219 ICC patients grouped by the median value of CXCL12 H-score in TMA cohort. I Bone-marrow-derived dendritic cells (BMDCs) stimulated with recombinant murine GM-CSF (20 ng/ml) and IL-4 (10 ng/ml) for 7 days. J Transwell migration assay of BMDCs treated by condition medium and CXCL12 (50 ng/ml), with or without IT1t dihydrochloride (20 µM) for 24 h. K qPCR analysis of Cxcr4 in DCs treated by CXCL12 in a concentration-dependent manner. **P < 0.01, ***p < 0.001, ****p < 0.0001.

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